Journal: Cell Communication and Signaling : CCS

Article Title: Ficolin-A induces macrophage polarization to a novel pro-inflammatory phenotype distinct from classical M1

doi: 10.1186/s12964-024-01571-4

Figure Lengend Snippet: FCNA treatment deteriorates CIA symptoms. A DBA/1 mice were immunized on Day0 and 21 to induce the CIA model, with the treatment of FCNA (5 µg/mouse) i.p. once every two days. B Arthritis scores are measured every two days (Control, n = 8; FCNA, n = 8). C Representative images of forelimbs and hindlimbs from different mice on Day42 post immunization. D The morphology of articular cartilage and synovial invasion were observed by H&E staining, safranine O-fast green staining and toluidine blue staining of mice ankle joints. Scale bars, 400 μm. E and F Quantification of H&E staining by HSS scores and OARSI scores ( n = 8/group). The P value was determined by two-way ANOVA ( B ) and unpaired Student’s t test ( E, F ). Error bars show the mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001

Article Snippet: 4 μm paraffin sections were prepared from sagittal view with a paraffin microtome (Leica, Wetzlar, Hessen, Germany), and stained with Modified Safranine O-Fast Green FCF Cartilage Stain Kit (Solarbio, Wuhan, China).

Techniques: Staining

Journal: Cell Communication and Signaling : CCS

Article Title: Ficolin-A induces macrophage polarization to a novel pro-inflammatory phenotype distinct from classical M1

doi: 10.1186/s12964-024-01571-4

Figure Lengend Snippet: Deletion of FCNA attenuates CIA symptoms. A Arthritis scores are measured every two days (WT, n = 6; Fcna-/-, n = 8) after the induction of CIA model. B Representative images of forelimbs and hindlimbs from different mice on Day42 post immunization. C The morphology of articular cartilage and synovial invasion were observed by H&E staining, safranine O-fast green staining and toluidine blue staining of mice ankle joints. Scale bars, 400 μm. D and E Quantification of H&E staining by HSS scores and OARSI scores ( n = 6/group). The P value was determined by two-way ANOVA and unpaired Student’s t test. Error bars show the mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001

Article Snippet: 4 μm paraffin sections were prepared from sagittal view with a paraffin microtome (Leica, Wetzlar, Hessen, Germany), and stained with Modified Safranine O-Fast Green FCF Cartilage Stain Kit (Solarbio, Wuhan, China).

Techniques: Staining